Wound dressings on the basis of seaweed polysaccharides, promising for the treatment of burn wounds

INTRODUCTION. Severe burn injury or burn disease is one of the most dangerous injuries to life. Treatment of burns, as well as subsequent rehabilitation of the burn patients are a serious problem for both civil health care and military medicine. The key component of local treatment of burn wounds is the use of wound dressings. Among the wide variety of used wound dressings the most promising are hydrogel based on seaweed polysaccharides (PS).

OBJECTIVE. Based on the analysis of data from domestic and foreign literature, to justify the use of seaweed polysaccharides (PS) as a basis or as therapeutic components of promising wound dressings for the treatment of wounds and burns.

MATERIALS AND METHODS. The articles included in the databases and information systems are analyzed: the scientific electronic library Elibary.ru, RISC, Web of Science, Pubmed, Scopus, Elsevier and Google Scholar (as of February 2023) by keywords: marine medicine, burn wounds, seaweed polysaccharides, wound dressings, hydrogels

RESULTS. The review presents information about modern biodegradable and biocompatible wounded dressings that have been used in clinical practice or are at the stage of experimental research and developed using seaweed PS (alginates and fucoidans of brown algae, carraginans of red algae, ulvanas of green algae) as a basis or as therapeutic components. A brief characteristic of the physicochemical properties and high biological activity of the PS, important both for macroorganism as a whole and for wounds healing is presented.

DISCUSSION. The use of PS when constructing wound dressings is justified, and the results of their experimental and clinical trials for the treatment of wounds and burns are analyzed. Particular attention is paid to the ability of the PS to form hydrogel, since hydrogel dressings meet the main requirements for the ideal wound dressings for the treatment of wounds of various genesis, including burn wounds.

CONCLUSION. Seaweed PS are widely used in modern technologies for creating wound dressings for the treatment of burn wounds.

marine medicine, burn wounds, seaweed polysaccharides, wound dressings, hydrogels

1. Государственный доклад «О состоянии защиты населения и территорий Российской Федерации от чрезвычайных ситуаций природного и техногенного характера в 2020 году». М.: МЧС России. ФГБУ ВНИИ ГОЧС (ФЦ), 2021. 264с. Протокол от 19 марта 2021 г. № 1 [The state report “On the state of the population and territories of the Russian Federation protection from emergency situations of natural and technogenic nature in 2020”. Moscow: Ministry of Emergency Situations of Russia. Federal State Budgetary Institution, 2021. 264 p. Protocol of March 19, 2021 № 1 (In Russ.)].

2. Bilal M., Iqbal H.M.N. Marine Seaweed Polysaccharides-Based Engineered Cues for the Modern Biomedical Sector. Mar Drugs, 2020, Vol. 18, No. 1, Р. 7. doi:10.3390/md18010007

3. de Jesus Raposo M.F., de Morais A.M., de Morais R.M. Marine polysaccharides from algae with potential biomedical applications. Mar Drugs, 2015, Vol. 13, No. 5, pp. 2967–3028. doi:10.3390/md13052967

4. Ovington L. The Evolution of Wound Management. Home Healthcare Nurse, 2020, Vol. 20, No. 10 Р.652-656. doi: 10.1097/00004045-200210000-00009

5. Park J.-H., Choi S.-H., Park S.-J., et al. Promoting Wound Healing Using Low Molecular Weight Fucoidan in a Full-Thickness Dermal Excision Rat Model. Mar. Drugs, 2017, Vol. 15, P. 112. doi: 10.3390/md150401125

6. Pozharitskaya O.N., Shikov A.N., Obluchinskaya E.D., Vuorela H. The pharmacokinetics of fucoidan after topical application to rats. Mar Drugs, 2019, Vol. 17, P. 687. doi: 10.3390/md17120687

7. Fitton H.J., Stringer D.S., Park A.Y., Karpiniec S.N. Therapies from Fucoidan: New Developments. Mar Drugs, 2019, Vol. 17, No. 10, Р. 571. doi:10.3390/md17100571.

8. Wang L., Lee W., Oh J.Y., et al. Protective Effect of Sulfated Polysaccharides from Celluclast-Assisted Extract of Hizikia fusiforme Against Ultraviolet B-Induced Skin Damage by Regulating NF-κB, AP-1, and MAPKs Signaling Pathways In Vitro in Human Dermal Fibroblasts. Mar Drugs, 2018, Vol. 16, P. 239. doi: 10.3390/md16070239

9. Pielesz A. Temperature-dependent FTIR spectra of collagen and protective effect of partially hydrolysed fucoidan. Spectrochim. Acta A Mol. Biomol. Spectrosc, 2014, Vol. 118, Р. 287–293. doi: 10.1016/j.saa.2013.08.056

10. Murakami K., Ishihara M., Aoki H., et al. Enhanced healing of mitomycin C-treated healing-impaired wounds in rats with hydrosheets composed of chitin/chitosan, fucoidan, and alginate as wound dressings. Wound Repair Regen, 2010, Vol. 18, pp. 478–485. doi: 10.1111/j.1524-475X.2010.00606.x

11. Yanagibayashi S., Kishimoto S., Ishihara M., et al. Novel hydrocolloid-sheet as wound dressing to stimulate healing-impaired wound healing in diabetic db/db mice. Biomed. Mater. Eng., 2012, Vol. 22, pp. 301–310. doi: 10.3233/BME-2012-0720

12. O’Leary R., Rerek M., Wood E.J. Fucoidan modulates the effect of transforming growth factor (TGF)-beta1 on fibroblast proliferation and wound repopulation in in vitro models of dermal wound repair. Biol Pharm Bull., 2004, Vol. 27, No. 2, pp. 266–270. doi: 10.1248/bpb.27.266

13. Kordjazi M., Shabanpour B., Zabihi E. et al. Sulfated polysaccharides purified from two species of padina improve collagen and epidermis formation in the rat. Int J Mol Cell Med, 2013, Vol. 2, No. 4, pp. 156–163. http://ijmcmed.org/article-1-103-en.html

14. Murakami K., Aoki H., Nakamura S., et al. Hydrogel blends of chitin/chitosan, fucoidan and alginate as healing-impaired wound dressings. Biomaterials, 2010, Vol. 31, No. 1, pp. 83–90. doi: 10.1016/j.biomaterials.2009.09.031

15. Sezer A.D., Cevher E., Hatıpoğlu F., et al. Preparation of fucoidan-chitosan hydrogel and its application as burn healing accelerator on rabbits. Biol. Pharm. Bull, 2008, Vol. 31, pp. 2326–2333. doi: 10.1248/bpb.31.2326

16. Feki A., Bardaa S., Hajji S., et al. Falkenbergia rufolanosa polysaccharide-poly (vinyl alcohol) composite films: A promising wound healing agent against dermal laser burns in rats. Int J Biol Macromol, 2020, Vol. 144, pp. 954–966. DOI:10.1016/j.ijbiomac.2019.09.173

17. Aderibigbe B., Buyana B. Alginate in Wound Dressings. Pharm, 2018, Vol. 10, No. 2, pp. 42. doi:10.3390/pharmaceutics10020042

18. Boateng J., Burgos-Amador R., Okeke O., Pawar H. Composite alginate and gelatin-based bio-polymeric wafers containing silver sulfadiazine for wound healing. Int. J. Biol. Macromol, 2015, Vol. 79, pp. 63–71. doi: 10.1016/j.ijbiomac.2015.04.048

19. Stoica A.E., Chircov C., Grumezescu A.M. Nanomaterials for Wound Dressings: An Up-to-Date Overview. Molecules, 2020, Vol. 25, No. 11. E2699. doi: 10.3390/molecules25112699

20. McBride C. A., Kimble R. M., Stockton K. A. Prospective randomised controlled trial of Algisite™ M, Cuticerin™, and Sorbact® as donor site dressings in paediatric split-thickness skin grafts. Burns Trauma, 2018, Vol. 6, pp. 33. doi:10.1186/s41038-018-0135-y

21. Rezvanian M., Amin M.C., Ng S.F. Development and physicochemical characterization of alginate composite film loaded with simvastatin as a potential wound dressing. Carbohydr. Polym, 2016, Vol. 137, pp. 295–304. doi: 10.1016/j.carbpol.2015.10.091

22. Catanzano O., D’Esposito V., Acierno S., et al. Alginate-hyaluronan composite hydrogels accelerate wound healing process. Carbohydr Polym, 2015, Vol. 131, pp. 407–414. doi: 10.1016/j.carbpol.2015.05.081

23. Xie H., Chen X., Shen X., et al. Preparation of chitosan-collagen-alginate composite dressing and its promoting effects on wound healing. Int. J. Biol. Macromol, 2018, Vol. 107, pp. 93–104. doi: 10.1016/j.ijbiomac.2017.08.142

24. Yegappan R, Selvaprithiviraj V, Amirthalingam S, Jayakumar R. Carrageenan based hydrogels for drug delivery, tissue engineering and wound healing. Carbohydr Polym, 2018, Vol. 198, pp. 385–400. doi: 10.1016 / j. carbpol.2018.06.086

25. Zepon K.M., Martins M.M., Marques M.S., et al. Smart wound dressing based on κ-carrageenan/locust bean gum/cranberry extract for monitoring bacterial infections. Carbohydr Polym, 2019, Vol. 206, pp. 362–370. doi: 10.1016/j.carbpol.2018.11.014

26. Lokhande G., Carrow J.K., Thakur T., et al. Nanoengineered injectable hydrogels from kappa-carrageenan and two-dimensional nanosilicates for wound healing application. Acta Biomaterialia, 2018, Vol. 70, pp. 35–47. doi: 10.1016/j.actbio.2018.01.045

27. Tytgat L., Van Damme L., Ortega Arevalo M.D.P., et al. Extrusion-based 3D printing of photo-crosslinkable gelatin and κ-carrageenan hydrogel blends for adipose tissue regeneration. Int J Biol Macromol, 2019, Vol. 140, pp. 929–938. doi: 10.1016/j.ijbiomac.2019.08.124

28. Li J., Yang B., Qian Y., et al. Iota-carrageenan/chitosan/gelatin scaffold for the osteogenic differentiation of adipose-derived MSCs in vitro. J Biomed Mater Res B Appl Biomater, 2015, Vol. 103, No. 7, pp. 1498–1510. doi: 10.1002/jbm.b.33339.

29. El-Fawal G.F., Yassin A.M., El-Deeb N.M. The novelty in fabrication of polyvinyl alcohol/κ-Carrageenan hydrogel with Lactobacillus bulgaricus extract as antiinflammatory wound dressing agent. AAPS PharmSciTech, 2017, Vol. 18, No. 5, pp. 1605–1616. doi: 10.1208/s12249-016-0628-6

30. Niar A.V., Raman M., Doble M. Cyclic β-(1→3) (1→6) glucan/carrageenan hydrogels for wound healing applications. RSC Advances, 2016, Vol.33, No. 3, pp. 98545–98553. doi:10.1039/C6RA23386D

31. Tziveleka L.A., Ioannou E., Roussis V. Ulvan, a bioactive marine sulphated polysaccharide as a key constituent of hybrid biomaterials: A review. Carbohydr Polym, 2019, Vol. 218, pp. 355–370. doi: 10.1016/j.carbpol.2019.04.074

32. Kanno K., Akiyoshi K., Nakatsuka T., et al. Biocompatible hydrogel from a green tide-forming chlorophyta. J. Sustainable Dev, 2012, Vol. 5, No. 4, pp. 38–45.

33. Toskas G., Hund R.D., Laourine E., et al. Nanofibers based on polysaccharides from the green seaweed Ulva rigida. Carbohydr. Polym, 2011, Vol. 84, pp. 1093–1102. doi: 10.1016/j.carbpol.2012.04.045

34. Kidgell J.T., Magnusson M., de Nys R., Glasson C.R.K. Ulvan: A systematic review of extraction, composition and function. Algal Research, 2019, Vol. 39, pp. 101422. doi: 10.1016/j.algal.2019.101422.

35. Glassonaian C.R.K., Sims I.M., Carnachan S.M., et al. A cascading biorefinery process targeting sulfated polysaccharides (ulvan) from Ulva ohnoi. Algal Research, 2017, Vol. 27, pp. 383–391. doi: 10.1016/j.algal.2017.07.001

36. Nardelli A.E., Chiozzini V.G., Braga E.S., et al. Integrated multi-trophic farming system between the green seaweed Ulva lactuca, mussel, and fish: a production and bioremediation solution. J Appl Phycol, 2019, Vol. 31, pp. 847–856. doi: 10.1007/s10811-018-1581-4

37. Adrien A., Bonnet A., Dufour D., et al. Pilot production of ulvans from Ulva sp. and their effects on hyaluronan and collagen production in cultured dermal fibroblasts. Carbohydr Polym, 2017, Vol. 157, pp. 1306–1314. doi: 10.1016/j.carbpol.2016.11.014

38. Jovic T.H., Kungwengwe G., Mills A.C., Whitaker I.S. Plant-Derived Biomaterials: A Review of 3D Bioprinting and Biomedical Applications. Front. Mech. Eng., 2019, Vol. 5, pp. 9. doi: 10.3389/fmech.2019.00019

39. Zhang L., Ma Y., Pan X., et al. A composite hydrogel of chitosan/heparin/poly (γ-glutamic acid) loaded with superoxide dismutase for wound healing. Carbohydr Polym. 2018, Vol. 180, pp. 168–174. doi: 10.1016/j.carbpol.2017.10.036

40. Vowden K., Vowden P. Wound dressings: principles and practice. Surgery (Oxford), 2017, Vol. 35, Issue 9, pp. 489–494. doi: 10.1016/j.mpsur.2017.06.005